Science
CRISPR gRNA & Off-Target Designer
SpCas9 · SaCas9 · Cas12a · base + prime editors with CFD scoring.
Live
Paste a target DNA sequence and pick a nuclease (SpCas9 NGG, SaCas9 NNGRRT, Cas12a TTTV, ABE/CBE base editors, prime editor). SciVue scans both strands, scores guides with a Doench-inspired heuristic, and searches a reference for off-targets using CFD scoring with position-weighted mismatch penalties. Genome browser track + BED export.
Five nuclease classes
SpCas9, SaCas9, Cas12a, base editors (ABE/CBE), and prime editors in one tool.
CFD off-targets
Doench 2016 position-weighted mismatch model up to N mismatches.
Base editor window
Predicts A→G or C→T edits inside the 4–8 nt activity window.
Who it's for
- Knockout / knock-in design
- Base editor screens
- CRISPR figure supplements
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