Science
Flow Cytometry & CyTOF
FCS 3.1 · asinh / biexp · polygon gating · UMAP + FlowSOM.
Live
Drop an FCS 3.1 file (or use the demo dataset). Apply asinh (cofactor 5 for CyTOF, 150 for fluorescence flow) or biexponential transforms, draw polygon gates by clicking on the plot, render dot / density / contour / histogram views, and run UMAP + FlowSOM-style k-means clustering on the gated subset.
Polygon gating
Click to add vertices, close the gate, sequential AND-gating across stages.
UMAP + FlowSOM
Embed thousands of events and cluster them with one button.
Viridis density
Log-scaled density plots ready for figures.
Who it's for
- Flow / CyTOF supplements
- Subpopulation discovery
- Cytometry teaching
More in Science