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Mutational Signatures & TMB

VCF/MAF → SBS-96 profile + COSMIC v3 signature decomposition.

Live

Parse a VCF or MAF, build the SBS-96 trinucleotide profile in the canonical pyrimidine orientation, and decompose against a COSMIC v3-style reference set (SBS1/3/4/6/7a/13) via multiplicative-update NNLS. Reports cosine similarity, per-signature exposures, and TMB (mut/Mb) for exome or WGS targets.

Canonical SBS-96 plot

Six pyrimidine substitution panels × 16 trinucleotide contexts.

Cosine + exposures

Reconstruction quality and per-signature exposures shown side by side.

TMB built in

Switch between exome (30 Mb) and WGS (3 Gb) targets in one click.

Who it's for
  • Cancer signature figures
  • Immunotherapy biomarker panels (TMB)
  • Mutational aetiology supplements
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